The study of titanium dioxide modification by glutaraldehyde and its application of immobilized penicillin acylase

Publication date: Available online 5 October 2018Source: Colloids and Surfaces A: Physicochemical and Engineering AspectsAuthor(s): Xudong Wang, Zhangjun Chen, Ke Li, Xiaodong Wei, Zhenbin Chen, Juan M. Ruso, Zhenghua Tang, Zhen LiuAbstractIn this work, titanium dioxide particle was firstly modified with glutaraldehyde, and modification conditions, i.e. particle size (d), active temperature (Ta), modification temperature (Tm ), modification time (tm), pH value, aldehyde ethanol ratio (Ra) and ethanol ratio (Re), were studied and optimized adopting grafting rate (Gr) as the optimizing index, the ultraviolet-visible spectrophotometry as characterization method, and the obtained optimal modification conditions were d = 3.3 µm, Ta = 120 °C, Tm = 45 °C, tm = 30 h, pH = 6.0, Ra = 1:10 and Re = 100%. Under the optimal conditions, Gr of glutaraldehyde arrived at 50%. Thereafter, a series of modified titanium dioxide with Gr in the range of [8%, 46%] and with Gr = 30% obtained from different modification conditions were adopted to study the immobilization of penicillin G acylase (PGA), during this process, the ultraviolet-visible spectrophotometry of 6-aminopenicillanic acid was conscripted as characterization method, the enzyme loading capacity (ELC), the enzyme activity (EA) and enzyme activity retaining ratio (EAR) were explored, and results documented with the increase of Gr, ELC, EA and EAR increased. As Gr kept as a similar level, ELC, ...

Source: Colloids and Surfaces A: Physicochemical and Engineering Aspects - October 6, 2018 Category: Chemistry Source Type: research