Inhibition of Nf- ҝb Prevents Trauma-induced Heterotopic Ossification in Rat Model.

Inhibition of Nf-ҝb Prevents Trauma-induced Heterotopic Ossification in Rat Model.
Connect Tissue Res. 2018 Oct 05;:
Authors: Ju J, Yu D, Xue F, Zhao Y, Shi W, Pan M, Tang G, Xiao H
Abstract
Acquired heterotopic ossification (HO), the ectopic formation of bone in soft tissues, is a common cause of joint pain and confined motion. The pathogenesis of HO must be better understood to find a better prophylactic method. In the first part, we designed the brain-traumatic/burn/tenotomy rat model and testified its efficacy as HO model. 44 rats were randomly divided into experimental group and control group. After operation, the bilateral tendons of 2 rats were collected at the 2nd, 3rd, 4th, 6th, 8th, and 10th weeks to determine the expression levels of p65. Additionally, the remaining rats were exposed to X-Ray examination at the 10th week. In the second part, 124 rats were randomly divided into four groups based on the administration dosage of Ammonium pyrrolidinedithiocarbamate (PDTC).Then, three rats of each group were euthanized every week in the first seven weeks to collect tendon to detect the expression levels of p65 by qRT-PCR and Western Blot. The remaining rats were exposed to X-Ray examination at the 10th week to assess the size of HO before being euthanized for HE staining. The success rate of Brain-traumatic/Burn/Tenotomy model was 100%. Pharmacologic inhibition of Nf-ҝb signaling pathway by PDTC could significantly reduce the...

Source: Connective Tissue Research - October 6, 2018 Category: Research Tags: Connect Tissue Res Source Type: research