GSE120892 Altered binding of tumor antigenic peptides to MHC class I affects CD8 T cell effector responses

Contributors : Eleanor Clancy-Thompson ; Christine A Devlin ; Paul M Tyler ; Mariah M Servos ; Lestat R Ali ; Katherine S Ventre ; M A Bhuiyan ; Patrick T Bruck ; Michael E Birnbaum ; Stephanie K DouganSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusT cell priming occurs when a na ïve T cell recognizes cognate peptide-MHC complexes on an activated antigen presenting cell. The circumstances of this initial priming have dramatic ramifications on the fate of the newly primed T cell. Newly primed CD8 T cells can embark onto different trajectories, with some becoming short lived effector cells and others adopting a tissue resident or memory cell fate. To determine whether T cell priming influences the quality of the effector T cell response to tumors, we used transnuclear CD8 T cells that recognize the melanoma antigen TRP1 using TRP1high or TRP1low TCRs that differ in both affinity and fine specificity. From a series of altered peptide ligands, we identified a point mutation (K8) in a non-anchor residue that, when analyzed crystallographically and biophysically, destabilizes the peptide interaction with the MHC binding groove. We then compared the transcriptional pro files of TRP1-specific T cells activated in vitro with M9 peptide to those activated with K8 peptide.
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research