A dose-dependent effect of dimethyl sulfoxide on lipid content, cell viability and oxidative stress in 3T3-L1 adipocytes

Publication date: Available online 4 October 2018Source: Toxicology ReportsAuthor(s): Phiwayinkosi V. Dludla, Babalwa Jack, Amsha Viraragavan, Carmen Pheiffer, Rabia Johnson, Johan Louw, Christo J.F. MullerAbstractDimethyl sulfoxide (DMSO) is an effective solvent and cytoprotectant agent that can induce diverse actions in experimental settings, ranging from metabolic stress to cytotoxic effects depending on concentration used. Therefore, for the quality of experiments and reproducibility of results it is essential to establish a precise and non-toxic dose of DMSO within a specific cell system. 3T3-L1 adipocytes, represent a well-established in vitro cell model used to assess the anti-obesity potential of extracts and compounds. Although DMSO is commonly used as a solvent for these experiments, there is limited data available on the compounding effects of using DMSO. The purpose of this study was to assess a concentration-dependent effect of DMSO on lipid content, cell viability and oxidative stress markers on 3T3-L1 adipocytes. Results showed that DMSO at doses
Source: Toxicology Reports - Category: Toxicology Source Type: research

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