Ionic liquid-based salt-induced liquid-liquid extraction of polyphenols and anthraquinones in Polygonum cuspidatum

In this study, ionic liquid was used as extraction solvent. The dried P. cuspidatum samples purchased from the pharmacy were triturated and passed through a 120-mesh sieve. The obtained sample powders were dried to constant weight at 55 ℃, and then mixed with extraction solvent. The extraction was carried out with the aid of ultrasound. Three phases, including ionic liquid-rich, salt-rich and solid sample phases were formed in the presence of salt. The target analytes were enriched in ionic liquid phase and then determined by high performance liquid chromatography. The experimental parameters, such as type and volume of ionic liquid, type and amount of salt, pH value of extraction medium, ultrasound power, ultrasound time and centrifugal condition, were optimized. The calibration curves showed good linear relationship (r> 0.9994). The limits of detection and quantification were in the range of 2.8–29.5 and 9.4–98.3 ng mL−1, respectively. The spiked recoveries were between 92.16% and 105.41%. Compared with hot reflux extraction and ultrasound-assisted extraction, the proposed method requires less extraction solvent and time. The present method can be applied to the determination of polyphenols and anthraquinones in P. cuspidatum.
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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CONCLUSIONS: Intra-observer reproducibility for thyroid nodule US reporting and US classification systems appears fairly adequate while the inter-observer agreement between different centers is lower than that assessed in single-center trials. Reporting and rating ability of thyroid US examiners still appear not consistent. An unified lexicon of thyroid US features, a simplified method of classification, and a dedicated training in the description of thyroid US findings may increase the observers' agreement and the predictive value of US classification systems in real world practice. PMID: 31952456 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
Conclusions: Active surveillance is possible in developing western countries with similar results to those provided by Asian and American authors. PMID: 31950887 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
Conclusion: Following an initial non-diagnostic FNAB, the probability of yielding a diagnostic result declines with each sequential repeat FNAB. Nonetheless, a tangible possibility of malignancy remains even after repeated non-diagnostic FNABs.  . PMID: 31950884 [PubMed - as supplied by publisher]
Source: Thyroid : official journal of the American Thyroid Association - Category: Endocrinology Tags: Thyroid Source Type: research
Publication date: Available online 18 January 2020Source: Journal of the Formosan Medical AssociationAuthor(s): Ching-Kai Lin, I-Shiow Jan, Kai-Lun Yu, Lih-Yu Chang, Hung-Jen Fan, Yueh-Feng Wen, Chao-Chi HoBackground/PurposeRapid on-site cytologic evaluation (ROSE) has been shown to improve the diagnostic accuracy of endobronchial ultrasound-guided transbronchial biopsy (EBUS-TBB). However, ROSE by a cytopathologist or cytotechnologist is not always available during the procedure. The purposes of this study were to verify that a pulmonologist, after receiving training in cytology, could accurately assess an EBUS-TBB specim...
Source: Journal of the Formosan Medical Association - Category: General Medicine Source Type: research
Publication date: February 2020Source: Annals of Emergency Medicine, Volume 75, Issue 2Author(s): Brit Long, Michael D. April, Alex Koyfman
Source: Annals of Emergency Medicine - Category: Emergency Medicine Source Type: research
Publication date: February 2020Source: Annals of Emergency Medicine, Volume 75, Issue 2Author(s): Michael Gottlieb, Stephen Alerhand
Source: Annals of Emergency Medicine - Category: Emergency Medicine Source Type: research
Authors: Crapé L, Strubbe B, Cesmeli E, de Clerck F Abstract A 24-year-old male presented with abdominal pain, postprandial vomiting and weight loss. Lab results showed an elevated serum eosinophil count and CT-scan demonstrated a thickened antral, duodenal and jejunal wall. Repetitive endoscopic mucosal biopsies were normal. Work-up of eosinophilia-associated gastro-intestinal disorders excluded secondary causes. Bone marrow showed an elevated eosinophil count without arguments for a primary hypereosinophilic syndrome. Endoscopic ultrasound-guided fine needle biopsy detected a strongly elevated number of eo...
Source: Acta Gastro-Enterologica Belgica - Category: Gastroenterology Tags: Acta Gastroenterol Belg Source Type: research
In this study, we investigated the benefits of the electrocardiogram (ECG)-based R wave pulse wave index (ERWVI) for the diagnosis of CA. MATERIAL AND METHODS According to CA examinations by color Doppler ultrasound, patients were assigned to positive and negative groups. The ECG R wave-Pulse wave transit time (ERWPTT) was obtained by synchronously collecting ECG signals (R wave in ECG) and the time variations in maximum finger pulse oxygen (DOP) on the ECG monitor. RESULTS ERPWI was positively correlated with sex, age, BMI, diastolic/systolic blood pressure, fasting blood glucose, uric acid, cholesterol and triglyceride l...
Source: Medical Science Monitor - Category: Research Tags: Med Sci Monit Source Type: research
We report a case of a 15-year-old girl who presented acute severe pain, a tenderness and a palpable mass in the left upper abdominal quadrant in a context of known ovarian cyst. An ultrasound described an ovarian cyst with uncertain vascularization. An urgent laparoscopy showed us a normal left ovary and revealed a very unusual combination of isolated fallopian tube torsion, with a big fallopian cyst and an elongated tube folded over the mesosigmoid. We performed a cystectomy without salpingectomy. This case reminds that paraadnexal and adnexal cysts can be found outside the pelvic area especially in children.
Source: Journal of Pediatric Surgery Case Reports - Category: Surgery Source Type: research
ConclusionsA nomenclature of 54 actions in Rheumatology was compiled. Biopsies (bone, sural nerve, synovial), inpatient visits, visits to the patient's home, infiltrations in children, and the preparation of the expert report were identified as the most complex actions. Musculoskeletal ultrasound is twice as complex as subsequent visits, capillaroscopy or bone densitometry.ResumenIntroducciónUna misión de la Sociedad Española de Reumatología es aportar las herramientas necesarias para alcanzar la excelencia asistencial. En la actualidad no existe una referencia que cuantifique la complejidad de ...
Source: Reumatologia Clinica - Category: Rheumatology Source Type: research
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