Determination of total and free ceftolozane and tazobactam in human plasma and interstitial fluid by HPLC-UV
Publication date: 30 January 2019Source: Journal of Pharmaceutical and Biomedical Analysis, Volume 163Author(s): Alexander Kratzer, Selina Schießer, Peter Matzneller, Beatrix Wulkersdorfer, Markus Zeitlinger, Jens Schlossmann, Frieder Kees, Christoph DornAbstractCeftolozane/tazobactam is a new cephalosporin/beta-lactamase inhibitor combination. An HPLC-UV method is described for the determination of total and free ceftolozane and tazobactam in human plasma and in microdialysate of subcutaneous tissue, respectively. Separation was performed using a reversed-phase column with phosphate buffer/acetonitrile as eluent and photometric detection at 260 nm (ceftolozane) or 220 nm (tazobactam). Linearity has been shown down to ceftolozane/tazobactam 0.1/0.05 mg/L in plasma and 0.03/0.015 mg/L in saline, respectively. The plasma protein binding of both drugs as determined by ultrafiltration was less than 10%. Temperature, pH or relative centrifugation force (up to 3000 x g) had no significant impact on the protein binding. The method was applied to the determination of ceftolozane and tazobactam in plasma and interstitial fluid of healthy volunteers following intravenous infusion of ceftolozane/tazobactam 1.0/0.5 g.
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Review of "Atlas of Artifacts in Clinical Neurophysiology," by William O. Tatum, IV Atlas of Artifacts in Clinical Neurophysiology by William O. Tatum , IV, Demos Medical Publishing, 2018 , 419 pages. ISBN: 9780826169341 . E-book ISBN: 978-0826169358 . List Price: $185.00; Amazon New: $154.02; Amazon Used: $144.00; Kindle: $129.36. Neurodiagn J. 2019 Aug 23;:1-2 Authors: Clark-Bash RJ PMID: 31441708 [PubMed - as supplied by publisher]
Authors: Moosavian SP, Arab A, Mehrabani S, Moradi S, Nasirian M Abstract Background: Several studies have investigated the effect of omega-3 fatty acids and vitamin E on oxidative stress and inflammation, but their findings are inconsistent. The aim of this meta-analysis is to elucidate the overall effects of co-supplementation with omega-3 fatty acids and vitamin E on oxidative stress and inflammation. Methods: We searched titles, abstracts, and keywords of relevant articles indexed in PubMed, ISI, Scopus, and Google Scholar databases up to December 2018 to identify eligible RCT studies. Random effects model was...
Conclusion: These results provide a potential mechanism involving AMPK activation/ROS inhibition signaling pathway in osteogenic differentiation of BMSCs enhanced by resveratrol. It suggests that development of therapy towards ROS is an effective way for osteoporosis treatment. PMID: 31438780 [PubMed - in process]
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