MicroRNA Profiling of Salivary Duct Carcinoma Versus Her2/Neu Overexpressing Breast Carcinoma Identify miR-10a as a Putative Breast Related Oncogene

AbstractSalivary duct carcinomas (SDC) and Her2/Neu3-overexpressing invasive breast carcinomas (HNPIBC/IBC) are histologically indistinguishable. We investigated whether common histopathologic and immunophenotypic features of SDC and IBC are mirrored by a similar microRNA (miRNA) profile. MiRNA profiling of 5 SDCs, 6 IBCs Her2/Neu3+, and 5 high-grade ductal breast carcinoma in situ (DCIS) was performed by NanoString platform. Selected miRNAs andHOXA1 gene were validated by RT-PCR. We observed similar miRNA expression profiles between IBC and SDC with the exception of 2 miRNAs,miR-10a andmiR-142-3p, which were higher in IBC tumors. DCIS tumors displayed increased expression ofmiR-10a, miR-99a, miR-331-3p andmiR-335, and decreased expression ofmiR-15a, miR-16 andmiR-19b compared to SDC. The normal salivary gland and breast tissues also showed similar expression profiles. Interestingly,miR-10a was selectively increased in both IBC and normal breast tissue compared to SDC and normal salivary gland tissue. Moreover, our NanoString and RT-PCR data confirmed thatmiR-10a was upregulated in IBC and DCIS compared to SDC. Finally, we show downregulation ofHOXA1, amiR-10 target, in IBC tumors compared to normal breast tissue. Taken together, our data demonstrates that, based on miRNA profiling, SDC is closely related to HNPIBC. Our results also suggest thatmiR-10a is differentially expressed in IBC compared to SDC and may have potential utility as a diagnostic biomarker in synchronous or...
Source: Head and Neck Pathology - Category: Pathology Source Type: research