Age-Related Peculiarities of Cytogenetic Disorders in Synovial Cells of Knee Joints in Northern Siberian Residents with Arthritis of Various Etiologies due to Polymorphism of the Glutathione S-Transferase Gene GSTM1

AbstractThe frequency of cells with cytogenetic disorders in the synovial fluid cells of the knee joints was studied in patients of different age groups with chronic arthritis associated with Lyme borreliosis (CAALB) or posttraumatic arthritis (PTA) with respect to polymorphism in the glutathioneS-transferase geneGSTM1. One hundred thirty five residents of the north of Tomsk and Tyumen oblasts were included in the study; 68 of them suffered from CAALB, while PTA was detected in the remaining 67 patients comprising the control group. Our results indicate the presence of significant age-related differences in the frequency of cytogenetic disorders in the synovial fluid cells of the knee joints between the groups of young and elderly CAALB patients. An integrative estimation of clinical and cytogenetic indices in the group of elderly CAALB patients (carriers of the mutantGSTM1 (0/0) allele) as compared with other groups allows the conclusion that there are significant positive correlations between the severity of violation of the locomotor function of the joints and the number of the synovial fluid cells with chromosome 7 trisomy.
Source: Advances in Gerontology - Category: Geriatrics Source Type: research

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Septic arthritis is a dangerous medical condition associated with significant morbidity and mortality. However, the differential diagnosis can be broad with conditions that mimic this disease and require different evaluation and treatment. This narrative review presents the emergency medicine evaluation and management, as well as important medical conditions that may mimic this disease. Septic arthritis commonly presents with monoarticular joint pain with erythema, warmth, swelling, and pain on palpation and movement. Fever is present in many patients, though most are low grade. Blood testing and imaging may assist with th...
Source: Western Journal of Emergency Medicine - Category: Emergency Medicine Source Type: research
ConclusionThe evidence base of biologic markers for A-RLA is limited. However, a range of promising biomarkers have been identified. Cytokines and chemokines related to Th17 pathway together with a number of miRNAs species (miR-146a, miR-155 and let-7a) may be promising candidates in the prediction of A-RLA. A panel of multiple biomarkers may yield clinically relevant prediction of the possible resistance at the time of LA first diagnosis.FundingPublic Health Agency of Canada.
Source: Infectious Diseases and Therapy - Category: Infectious Diseases Source Type: research
In this study, we investigated the mechanisms that lead to the production of proinflammatory mediators by the murine macrophage cell line, RAW264.7, when these cells are exposed in vitro to recombinant Borrelia burgdorferi basic membrane protein A (rBmpA). Using antibody protein microarray technology with high-throughput detection ability for detecting 25 chemokines in culture supernatant the RAW264.7 cell culture supernatants at 12 and 24 h post-stimulation with rBmpA, we identified two chemokines, a monocyte chemoattractant protein-5 (MCP-5/CCL12) and a macrophage inflammatory protein-2 (MIP-2/CXCL2), both of which incre...
Source: International Journal of Medical Sciences - Category: Biomedical Science Tags: Int J Med Sci Source Type: research
The study compares diagnostic parameters of different commercial serological kits based on three different antigen types and correlates test results with the status of the patient's Borrelia infection. In total, 8 IgM and 8 IgG kits were tested, as follows: enzyme-linked immunosorbent assay (ELISA) (Euroimmun) based on whole-cell antigen, 3 species-specific enzyme immunoassays (EIAs) (TestLine), Liaison chemiluminescence (DiaSorin), ELISA-Viditest (Vidia), EIA, and Blot-Line (TestLine) using recombinant antigens. All tests were performed on a panel of 90 samples from patients with clinically characterized borreliosis (53 w...
Source: Journal of Clinical Microbiology - Category: Microbiology Authors: Tags: Immunoassays Source Type: research
Conditions:   Joint Infection;   Infection of Hip Joint (Disorder);   Infection of Shoulder Joint;   Septic Arthritis Interventions:   Diagnostic Test: Synovial Alpha-defensin assay;   Diagnostic Test: Synovial Neutrophil elastase assay;   Diagnostic Test: Synovial lactate assay;   Diagnostic Test: Synovial C-reactive Protein (CRP);   Diagnostic Test: Synovial Staphylococcus spp antigen panel;   Diagnostic Test: Sy novial Candida spp antigen panel;   Diagnostic Test: Synovial Enterococ...
Source: - Category: Research Source Type: clinical trials
Abstract Lyme disease (borreliosis) is a tick-borne bacterial infection caused by the spirochaete Borrelia burgdoferi, transmitted by hard-backed Ixodes ticks. Actual numbers of cases are increasing and it appears that the distribution across the UK is widening; however, it occurs most frequently in area of woodland, with temperate climate. It typically presents in mid to late summer. Lyme disease is a multisystem disease. The nervous system is the second most commonly affected system after the skin. Other systemic manifestations, such as carditis, keratitis, uveitis and inflammatory arthritis, rarely occur in Eur...
Source: Practical Neurology - Category: Neurology Authors: Tags: Pract Neurol Source Type: research
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Source: Arthritis and Rheumatology - Category: Rheumatology Authors: Source Type: research
Cellular Microbiology,Volume 0, Issue ja, -Not available-.
Source: Cellular Microbiology - Category: Microbiology Authors: Source Type: research
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Source: Inflammation Research - Category: Research Source Type: research
As Lyme disease and Lyme arthritis spread to new regions in North America, physicians may ned to become aware of their signs and symptoms. Allen C. Steere, MD, says “Lyme arthritis is more complicated to treat than other manifestations of the disease.” Here are some best practices for treating Lyme arthritis...
Source: The Rheumatologist - Category: Rheumatology Authors: Tags: Conditions best practices Borrelia burgdorferi Lyme arthritis Lyme Disease Source Type: research
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