Analysis of DFS70 pattern and impact on ANA screening using a novel HEp-2 ELITE/DFS70 knockout substrate

AbstractIndirect immunofluorescence (IIF) using human epithelial cell (HEp-2) substrate is a widely used and the recommended method for screening of antinuclear antibodies (ANA). Dense fine speckled (DFS70) pattern on HEp-2 has been widely reported in various healthy and disease groups. Interpretation of DFS70 pattern can be challenging on a conventional HEp-2 substrate due to its similarity to some of the disease associated patterns. The high prevalence of DFS70 autoantibodies in normal population, lack of association with a particular disease group and a general negative association with systemic and ANA associated autoimmune rheumatic diseases (SARD/AARD) necessitates the confirmation of DFS70 pattern. Results using available commercial assays for confirmation of DFS70 autoantibodies do not always agree with IIF screening results further complicating the lab work flow and ANA algorithms. In this review, we discuss the prevalence of DFS70 antibodies and factors affecting the performance of IIF and DFS70 specific confirmatory assays. Factors that contribute to disagreement between DFS70 suspicion by IIF and confirmatory assays will also be discussed. In addition, we also describe a novel IIF HEp-2 substrate, and its positive impact on DFS70 reporting and ANA screening-confirmation algorithm.
Source: Autoimmunity Highlights - Category: Allergy & Immunology Source Type: research

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This study aims to validate this observation. Blood levels of seven cytokines were measured in 120 patients with pSS from the United Kingdom Primary Sjögren’s Syndrome Registry and 30 age-matched healthy non-fatigued controls. Patient-reported scores for fatigue were classified acco rding to severity and compared to cytokine levels using analysis of variance. The differences between cytokines in cases and controls were evaluated using Wilcoxon test. A logistic regression model was used to determine the most important identifiers of fatigue. Five cytokines, interferon-γ-induced protein-10 (IP-10), tumour ne...
Source: Rheumatology International - Category: Rheumatology Source Type: research
AbstractWe read with great interest the letter of Xieet al commenting on our article “Safety and Efficacy of Immune Checkpoint Inhibitors in Patients with Cancer and Preexisting Autoimmune Disease: A Nationwide Multicenter Cohort Study”(1).First, the authors suggested classifying Preexisting Autoimmune Diseases (PAD) according to systems, in order to assess if rheumatic disorders were more likely to flare with ICI. Such trend has been reported in other series, like Menzieset al and Leonardiet al, with the limit that the majority of the PAD in their cohort was a rheumatic disorder (n=27, 52% and n=25 (45%) respectively) (2,3).
Source: Arthritis and Rheumatology - Category: Rheumatology Authors: Tags: REPLY TO LETTER TO THE EDITOR Source Type: research
AbstractWe read with great interest the article by Tison et al profiling safety and efficacy of immune checkpoint inhibitors (ICIs) in individuals with cancer and preexisting autoimmune disease (PAD) (1). In this nationwide multicenter cohort study including largest population after Kehl KL's report (2), the occurrence of the PAD flare/other immune related adverse events (irAE) was frequent but mostly manageable without ICIs discontinuation. However, some issues should be addressed. Firstly, previous studies based on relatively small sample size indicated there are differences of flare rate among PAD of different systems (...
Source: Arthritis and Rheumatology - Category: Rheumatology Authors: Tags: LETTER TO THE EDITOR Source Type: research
ConclusionOur study showed a significant association between alterations in intestinal microbial composition and T1D; however, in some articles, it is not clear which one happens first. Investigation of altered gut microbiota can help in the early detection of T1D before seropositivity. Targeted microbiome modulation can be a novel potential therapeutic strategy.
Source: Gut Pathogens - Category: Microbiology Source Type: research
Conclusions/interpretationOur study identifies a proinsulin-derived peptide region that is highly diabetogenic on the HLA-DR3-DQ2 background using an in vivo model. This approach and the peptide region identified may have wider implications for future studies of human type 1 diabetes.
Source: Diabetologia - Category: Endocrinology Source Type: research
by Alireza Tafazzol, Yong Duan Toll-like receptors (TLRs) play a central role in both the innate and adaptive immune systems by recognizing pathogen-associated molecular patterns and inducing the release of the effector molecules of the immune system. The dysregulation of the TLR system may cause various autoimmune diseases and septic shock. A series of molecular dynamics simulations and free energy calculations were performed to investigate the ligand-free, lipopolysaccharide (LPS)-bound, and neoseptin3-bound (TLR4-MD2)2 tetramers. Compared to earlier simulations done by others, our simulations showed that TLR4 structure...
Source: PLoS Computational Biology - Category: Biology Authors: Source Type: research
Publication date: Available online 9 October 2019Source: NeuroImageAuthor(s): Aline M. Thomas, Jiadi Xu, Peter A. Calabresi, Peter C.M. van Zijl, Jeff W.M. BulteAbstractMultiple sclerosis (MS) is an autoimmune disorder that targets myelin proteins and results in extensive damage in the central nervous system in the form of focal lesions as well as diffuse molecular changes. Lesions are currently detected using T1-weighted, T2-weighted, and gadolinium-enhanced magnetic resonance imaging (MRI); however, monitoring such lesions has been shown to be a poor predictor of disease progression. Chemical exchange saturation transfer...
Source: NeuroImage - Category: Neuroscience Source Type: research
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Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Publication date: Available online 11 October 2019Source: Life SciencesAuthor(s): Roza Motavalli, Jalal Etemadi, Houman Kahroba, Amir Mehdizadeh, Mehdi YousefiAbstractIdiopathic membranous nephropathy (IMN) has recently attracted much attention due to the development of auto antibodies, anti-phospholipase A2 receptor and anti-thrombospondin type I domain-containing 7A on podocytes, the establishment of immune networks complexes in circulation as well as the development of autoreactive immune cells against kidney, in both innate and adaptive participants. The auto inflammatory responses in IMN leads to the dysfunction of gl...
Source: Life Sciences - Category: Biology Source Type: research
AbstractIn recent years, mold has been blamed for many symptoms or a constellation of symptoms. These symptoms are usually vague and subjective and difficult or impossible to measure or quantify. Moreover, there is no scientific evidence that mold has anything to do with these symptoms. In particular, the concept of toxic mold syndrome has permeated the public consciousness, and mycotoxins have falsely been associated with autoimmune diseases and a variety of other conditions. In fact, there is no evidence that the presence of mycotoxins in the air is enough to cause any disease known to man. Molds legitimately can cause a...
Source: Clinical Reviews in Allergy and Immunology - Category: Allergy & Immunology Source Type: research
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