GSE73722 Comparative transcriptome analysis in induced neural stem cells reveals defined neural cell identities in vitro and after transplantation into the adult rodent brain

Contributors : Anna-Lena Hallmann ; Christina Zerfass ; Marcos J Ara úzo-Bravo ; Volker Senner ; Marc Ehrlich ; Olympia E Psithaki ; Don-Wok Han ; Natalia Tapia ; Holm Zaehres ; Hans R Schöler ; Tanja Kuhlmann ; Gunnar HargusSeries Type : Expression profiling by arrayOrganism : Mus musculusReprogramming technology enables the production of large quantities of neural progenitor cells (NPCs) from somatic cells by direct transdifferentiation.However, little is known on how neural programs in these neural stem cells (iNSCs) differ from those of alternative stem cell populations in vitro and in vivo.Here, we performed transcriptome analyses on iNSCs in comparison to brain-derived neural stem cells (NSCs) and pluripotent stem cell-derived NPCs, which revealed distinct global, neural, metabolic and cell cycle-associated marks in these populations. iNSCs carried a hindbrain/spinal cord identity, which could be shifted towards caudal, partially to rostral but not towards ventral fates in vitro. iNSCs survived after transplantation into the rodent brain and exhibited in vivo-characteristics, neural and metabolic programs similar to those of transplanted NSCs. However, iNSCs vastly retained caudal identities demonstrating cell-autonomy of regional programs in vivo.These data could have significant implications for a variety of biomedical applications using iNSCs.For microarray analysis, total RNA was isolated using the RNeasy kit (QIAGEN) according to the manufacturer ´s ins...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research