Oral immunization with a novel attenuated Salmonella Gallinarum encoding infectious bronchitis virus spike protein induces protective immune responses against fowl typhoid and infectious bronchitis in chickens

AbstractFowl typhoid (FT), a septicemic disease caused bySalmonella Gallinarum (SG), and infectious bronchitis (IB) are two economically important avian diseases that affect poultry industry worldwide. Herein, we exploited a live attenuated SG mutant, JOL967, to deliver spike (S) protein 1 of IB virus (V) to elicit protective immunity against both FT and IB in chickens. The codon optimized S1 nucleotide sequence was cloned in-frame into a prokaryotic constitutive expression vector, pJHL65. Subsequently, empty pJHL65 or recombinant pJHL65-S1 plasmid was electroporated into JOL967 and the resultant clones were designated as JOL2068 and JOL2077, respectively. Our results demonstrated that the chickens vaccinated once orally with JOL2077 elicited significantly (p 
Source: Veterinary Research - Category: Veterinary Research Source Type: research

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Emergency-use authorizations, a formerly obscure corner of regulatory law, have become a centerpiece of the government ’s response to the pandemic.
Source: NYT - Category: American Health Authors: Tags: your-feed-science Coronavirus Risks and Safety Concerns Food and Drug Administration Hahn, Stephen M (1960- ) Coronavirus (2019-nCoV) Vaccination and Immunization Clinical Trials Drugs (Pharmaceuticals) your-feed-health your-feed-healthc Source Type: news
The use of an immune-system stimulant harvested from shark liver oil in the development of some coronavirus vaccines has animal conservationists pressing for alternatives.(Image credit: Reinhard Dirscherl/ullstein bild via Getty Images)
Source: NPR Health and Science - Category: Consumer Health News Authors: Source Type: news
Authors: Abdeta D, Kebede N, Giday M, Terefe G, Abay SM Abstract Microbial resistance to the few conventional antitrypanosomal drugs, increasing resistance of vectors to insecticides, lack of effective vaccines, and adverse effects of the existing antitrypanosomal drugs justify the urgent need for effective, tolerable, and affordable drugs. We assessed antitrypanosomal effects of the hydromethanolic extract of Echinops kebericho Mesfin roots against Trypanosoma congolense field isolate using in vitro and in vivo techniques. Parasite load, packed cell volume (PCV), body weight, and rectal temperature in Swiss albino...
Source: Evidence-based Complementary and Alternative Medicine - Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Phoenix Children ’s Hospital held its fourth annual flu POD (point of distribution) this week, with this year's 13-hour blitz holding a special significance amid the global Covid-19 pandemic. On Oct. 7, nurses began vaccinating employees at 6 a.m. and concluded the one-day event at 7 p.m., vaccinating nearly 5,00 0 people. It’s preparation for another potential mass vaccination event: distribution of a safe and effective vaccine for Covid-19. Click through the photo gallery above to see scenes…
Source: bizjournals.com Health Care:Biotechnology headlines - Category: Biotechnology Authors: Source Type: news
Publication date: Available online 9 October 2020Source: Journal of Global Antimicrobial ResistanceAuthor(s): Elma L. Leite, Wydemberg J. Araújo, Tatiana R. Vieira, Karoline S. Zenato, Priscylla C. Vasconcelos, Samuel Cibulski, Patricia E.N. Givisiez, Marisa R.I. Cardoso, Celso J.B. Oliveira
Source: Journal of Global Antimicrobial Resistance - Category: Infectious Diseases Source Type: research
In this study, we modeled an alternative strategy to amplify tumor antigen-specific TCR transgenic CD8+ T cells through limited application of a long-acting IL-2 fusion protein, mIL-2/mCD25, which selectively targets the high-affinity IL-2R. Here, mice were vaccinated with a tumor antigen and high-dose mIL-2/mCD25 was applied to coincide with the induction of the high affinity IL-2R on tumor-specific T cells. A single high dose of mIL-2/mCD25, but not an equivalent amount of IL-2, amplified the frequency and function of tumor-reactive CD8+ T effector (Teff) and memory cells. These mIL-2/mCD25-dependent effects relied on di...
Source: Cancer Immunology, Immunotherapy - Category: Cancer & Oncology Source Type: research
In this study, we present a new method for the simultaneous detection of CXCR4, mesodermal markers and NCCs markers during chicken embryo developmental stages HH18 –HH25 by combining double whole-mount in situ hybridization (ISH) and immunostaining on floating vibratome sections. The simultaneous detection of CXCR4 and markers for the mesodermal and neural crest cells in multiple labelling allowed us to compare complex gene expression patterns and it could b e easily used for a wide range of gene expression pattern analyses of other chicken embryonic tissues. All steps of the procedure, including the preparation of p...
Source: Histochemistry and Cell Biology - Category: Biomedical Science Source Type: research
AbstractPurpose of ReviewLymphatic disorders have received an increasing amount of attention over the last decade. Sparked primarily by improved imaging modalities and the dawn of lymphatic interventions, understanding, diagnostics, and treatment of lymphatic complications have undergone considerable improvements. Thus, the current review aims to summarize understanding, diagnostics, and treatment of lymphatic complications in individuals with congenital heart disease.Recent FindingsThe altered hemodynamics of individuals with congenital heart disease has been found to profoundly affect morphology and function of the lymph...
Source: Current Cardiology Reports - Category: Cardiology Source Type: research
According to officials, the Hyderabad-based vaccine maker applied to the Drugs Controller General of India (DCGI) on October 2, seeking its permission to conduct phase-3 randomised double-blind placebo-controlled multicentre trial of its COVID-19 vaccine candidate.
Source: The Economic Times Healthcare and Biotech News - Category: Pharmaceuticals Source Type: news
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