Silymarin and curcumin effects on virulence and carbapenemase genes among multidrug-resistant Escherichia coli clinical isolates

Objective : Silymarin and curcumin antimicrobial properties have been previously described against some infectious agents. The aims of the current study were the investigation of the antibacterial effects of these compounds on the expression of fimA, cdt, blaIMP1 and blaOXA-48 and AcrAB-TolC genes among multidrug-resistant Escherichia coli clinical isolates. Methods : A total of five carbapenemase-producing E. coli strains were included. All of them carried blaIMP1 and blaOXA-48, ACR-AB, cdt, fimA and QepA genes. The antibiotic susceptibility test, imipenem, cefotaxime and ceftazidime minimum inhibitory concentration (MIC) and combine disk and Carba NP-test for carbapenemase production were performed. Silymarin and curcumin minimum inhibitory and bactericidal concentrations [MIC and minimum bactericidal concentration (MBC), respectively] were determined. The effect of 25 μg/ml concentration was also evaluated against gene expression. The quantitative real-time PCR was performed for the evaluation of genes expression. Results : Silymarin MIC and MBC were 256 and more than 256 μg/ml, respectively. Moreover, curcumin MIC and MBC concentrations were 128 and 256 μg/ml, respectively. Silymarin down-expressed the ACR-AB, cdt, fimA genes and QepA genes significantly (P 
Source: Reviews in Medical Microbiology - Category: Microbiology Tags: Bacteriology Source Type: research