Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration.

Repulsive Environment Attenuation during Adult Mouse Optic Nerve Regeneration. Neural Plast. 2018;2018:5851914 Authors: Goulart CO, Mendonça HR, Oliveira JT, Savoldi LM, Dos Santos Heringer L, Dos Santos Rodrigues A, Paes-de-Carvalho R, Martinez AMB Abstract The regenerative capacity of CNS tracts has ever been a great hurdle to regenerative medicine. Although recent studies have described strategies to stimulate retinal ganglion cells (RGCs) to regenerate axons through the optic nerve, it still remains to be elucidated how these therapies modulate the inhibitory environment of CNS. Thus, the present work investigated the environmental content of the repulsive axon guidance cues, such as Sema3D and its receptors, myelin debris, and astrogliosis, within the regenerating optic nerve of mice submitted to intraocular inflammation + cAMP combined to conditional deletion of PTEN in RGC after optic nerve crush. We show here that treatment was able to promote axonal regeneration through the optic nerve and reach visual targets at twelve weeks after injury. The Regenerating group presented reduced MBP levels, increased microglia/macrophage number, and reduced astrocyte reactivity and CSPG content following optic nerve injury. In addition, Sema3D content and its receptors are reduced in the Regenerating group. Together, our results provide, for the first time, evidence that several regenerative repulsive signals are reduced in regeneratin...
Source: Neural Plasticity - Category: Neurology Authors: Tags: Neural Plast Source Type: research