Selective detection of DNA of viable   Mycobacterium tuberculosis complex strains using EMA-PCR method.

In this study, the ability to discriminate viable cells from dead cells of Mycobacterium tuberculosis complex (MTC) using the viable bacteria selection kit for PCR (Gram Positive) (TaKaRa Bio, Inc., Japan), an Ethidium monoazide (EMA) treatment kit was examined. The range of bacterial concentrations in which the detection of dead bacteria was determined as 1.5 × 107-3.0 × 107 CFU/mL, which equivalent to McFarland No. 0.05-0.10. There was a significant difference between results of viable and dead bacteria, and the sensitivity and specificity of this method for culture-negative samples from patients were 83% and 100%, respectively. To the best of our knowledge, this is the first successful selective detection of DNA from viable cells of MTC by EMA-PCR using the viable bacteria selection kit for PCR (Gram Positive), an EMA treatment kit. We believe that application of this method could promote the earlier discharge of patients receiving tuberculosis treatment by discriminating dead cells from viable cells. PMID: 30270248 [PubMed - as supplied by publisher]
Source: Japanese Journal of Infectious Diseases - Category: Infectious Diseases Authors: Tags: Jpn J Infect Dis Source Type: research