Successful Secondary Engraftment of Pancreatic Ductal Adenocarcinoma and Cholangiocarcinoma Patient-Derived Xenografts After Previous Failed Primary Engraftment.

CONCLUSION: Secondary PDX engraftment using cryopreserved primary cancerous is feasible after previous failed engraftment attempts and can result in a 50% increase in overall engraftment efficiency with decreases in LT formation. This technique allows for salvage of critical patient PDX models that would otherwise not exist.
SYNOPSIS: Patient-derived xenografts have many important translational applications however can be limited by engraftment failure. We demonstrate optimized methodology utilizing cryopreservation of primary tumor tissue that allows for subsequent successful secondary engraftment and creation of PDX models that failed previous primary engraftment and allowed salvage of patient PDX models that would otherwise not exist.
PMID: 30273859 [PubMed - as supplied by publisher]

Source: Translational Oncology - September 28, 2018 Category: Cancer & Oncology Authors: Hernandez MC, Yang L, Leiting JL, Sugihara T, Bergquist JR, Ivanics T, Graham R, Truty MJ Tags: Transl Oncol Source Type: research