Taurolidine promotes cell apoptosis by enhancing GRIM ‑19 expression in liver cancer.

Taurolidine promotes cell apoptosis by enhancing GRIM‑19 expression in liver cancer. Oncol Rep. 2018 Sep 18;: Authors: Li F, Qi J, Qin C, Fu Z, Ren W Abstract Taurolidine (TRD) is a substance derived from the amino sulfonic acid taurine, which was originally used to treat peritonitis and catheter‑associated bloodstream infections, due to its antimicrobial and anti‑inflammatory properties. A recent study reported the anticancer function of TRD in malignant tumors; however, the effects and mechanisms of TRD in liver cancer remain unclear. The present study aimed to investigate the effects and mechanism of TRD treatment on human liver cancer cells. The viability and apoptosis of liver cancer cells were evaluated using the MTT assay and flow cytometry. Subsequently, small interfering RNA (siRNA) was used to knock down the expression of gene associated with retinoid‑interferon‑induced mortality‑19 (GRIM‑19), after which, reverse transcription‑quantitative polymerase chain reaction was used to detect the mRNA expression levels of GRIM‑19, whereas immunofluorescence was used to analyze the location of GRIM‑19. Furthermore, western blotting was performed to detect the protein expression levels of GRIM‑19, cyclin D1, signal transducer and activator of transcription 3 (STAT3), phosphorylated (p)‑STAT3, B‑cell lymphoma 2 (Bcl‑2) and Bcl‑2‑associated X protein (Bax). The STAT3 pathway was inhibited using niclo...
Source: Oncology Reports - Category: Cancer & Oncology Tags: Oncol Rep Source Type: research