Simple, rapid and sensitive portable molecular diagnosis of SFTS virus using Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP).

Simple, rapid and sensitive portable molecular diagnosis of SFTS virus using Reverse Transcriptional Loop-Mediated Isothermal Amplification (RT-LAMP). J Microbiol Biotechnol. 2018 Sep 20;: Authors: Baek YH, Cheon HS, Park SJ, Lloren KKS, Ahn SJ, Jeong JH, Choi WS, Yu MA, Kwon HI, Kwon JJ, Kim EH, Kim YI, Antigua KJC, Kim SY, Jeong HW, Choi YK, Song MS Abstract Recently, human infections with severe fever with thrombocytopenia syndrome virus (SFTSV) leading to fatality, have dramatically increased in East Asia. With the unavailability of vaccines or antiviral drugs to prevent and/or treat SFTSV infection, early rapid diagnosis is critical for prevention and control of the disease. Here, we report the development of a simple, rapid and sensitive portable detection method for SFTSV infection applying reverse transcription-loop mediated isothermal amplification (RT-LAMP) combined with one-pot colorimetric visualization and electro-free reaction platform. This method utilizes a pocket warmer to facilitate diagnosis in resource-limited setting. Specific primers were designed to target the highly conserved region of L gene of SFTSV. The detection limit of the RT-LAMP assay was approximately 100 viral genome copies from three different SFTSV strains. This assay exhibited comparable sensitivity to qRT-PCR and 10-fold more sensitivity than conventional RT-PCR, with a rapid detection time of 30 to 60 minutes. The RT-LAMP assay using SFTSV clini...
Source: Journal of Microbiology and Biotechnology - Category: Biotechnology Authors: Tags: J Microbiol Biotechnol Source Type: research