GSE112236 Cell type-specific endometrial transcriptome changes in comparison of equine endometrium samples collected on Day 12 of pregnancy and Day 12 of the estrous cycle

Contributors : Iside Scaravaggi ; Stefan BauersachsSeries Type : Expression profiling by high throughput sequencingOrganism : Equus caballusThe results of endometrial gene expression studies during the time of conceptus migration (Days 8 to 16) did not provide final conclusions on possible mechanisms of maternal recognition of pregnancy (MRP) in the mare. This finding called for the analysis of cell type-specific gene expression in the endometrium in response to the equine embryo, which could be the key for improved understanding of gene expression regulation in context of MRP. In a pilot study, we used laser capture microdissection (LCM) to collect samples of the luminal epithelium (LE), glandular epithelium (GE), and stroma from endometrial biopsies taken from five mares on Day 12 of pregnancy and Day 12 of the estrous cycle, respectively. RNA was isolated from the LCM samples in order to perform RNA sequencing. Data analysis showed that gene expression differences were greater between cell types than between pregnant and cyclic state. Differential gene expression between pregnant and cyclic state was mainly found in the LE but also in GE and stroma. The comparison with a previous RNA-Seq data set for whole biopsy samples derived from Day 12 of pregnancy revealed the specific origin of the observed gene expression differences. Furthermore, we found that a number of genes are specifically differentially expressed (DE) in only one cell type. Some genes not DE when looking a...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Equus caballus Source Type: research