GSE120289 Profiles analysis reveals circRNAs involving zebrafish physiological development

Contributors : Heng Liu ; Yin Hu ; Jing Yin ; Xiang-Yun Yan ; Wen-Juan Chen ; Xing-Yun Wang ; Zhang-Bin Yu ; Shu-Ping HanSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Danio rerioRecent studies have found that known functions of circRNAs include sequestration of microRNAs or proteins, modulation of transcription and interference with splicing, and even translation to produce poly-peptides. The zebrafish model is also demonstrably similar to humans in many studies. In order to explore the changes in circRNAs during embryonic development, further to research the mechanism of action of circRNAs in development-related diseases. Zebrafish embryos at blastula period, gastrula period, segmentation period, throat stage and incubation period were collected. Illumina deep sequencing technology and CIRI algorithm were used to detecting circRNAs. Totally we identified 1028 circRNAs (junction reads ≥ 5 and p< 0.05). Considering that circRNAs function is related to host genes, then bioinformatics analysis revealed these differentially expressed host genes are involved in NOTCH signaling pathways, cardiovascular system development, retinal ganglion cell axon guidance and so on. Moreover, circRNAs can participate in biological regulation through miRNA sponges function. TargetScan and miRanda were used to predict 73 miRNAs binding to circRNAs such as miR-19b, miR-124 and so on, some miRNAs play important roles in embryogenesis. The peak expression of ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Danio rerio Source Type: research