Directly observing alterations of morphology and mechanical properties of living cancer cells with atomic force microscopy.

Directly observing alterations of morphology and mechanical properties of living cancer cells with atomic force microscopy. Talanta. 2019 Jan 01;191:461-468 Authors: Wang N, Zhang M, Chang Y, Niu N, Guan Y, Ye M, Li C, Tang J Abstract Epithelial-mesenchymal transition (EMT) is a biological process during which cells lose their characteristic structure and biochemical properties then adopt typical features of a mesenchymal phenotype. Alterations in the morphology, structure, and mechanical properties of cells during EMT are associated with a series of pathological processes. In this work, atomic force microscopy (AFM) is used for investigating effects of TGF-β1 on morphology and mechanical properties of living bladder cancer cells (T24) during EMT for the first time. High-resolution topography and Young's modulus images of T24 living cell are obtained simultaneously. The results show that TGF-β1 is able to induce EMT, leading to the increased F-actin stress fibers and much higher Young's modulus values of T24 living cells. It reveals that the cytoskeletal-associated cell architecture is closely related to the mechanical dynamics of T24 cells during EMT. This work provides new insights into the changes of cell morphology and mechanical properties during EMT. It enables us to gain a deeper understanding of the growth, development and metastasis of the bladder cancer cell therefore it is of great significance for studying the pathologi...
Source: Talanta - Category: Chemistry Authors: Tags: Talanta Source Type: research