Bioanalytical method development and validation of Moxidectin in plasma by LC-MS/MS:application to in-vitro metabolism.

In this study, we developed and validated a LC-MS/MS method of MOX in human, monkey and mouse plasma. The separation was achieved on an ACE C18 (50 × 50 mm, 3μ) column with isocratic elution using 0.1% acetic acid and methanol:acetonitrile (1:1, v/v) as mobile phase. MOX was quantitated using MS/MS with electrospray ionization source operating in negative MRM mode. The MRM precursor ion → product ion transitions for MOX and abamectin (IS) were m/z 638.40→236.30 and m/z 871.50→565.35 respectively. The MS/MS response was linear over the concentration range from 0.1-1000 ng/mL in plasma with a correlation coefficient (r2 ) of 0.997 or better. The within- and between-day precision (relative standard deviation, % RSD) and accuracy were within the acceptable limits per FDA guidelines. The method was successfully applied to an in vitro metabolic stability study of MOX. PMID: 30238696 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research