Expression, purification of Zika virus membrane protein-NS2B in detergent micelles for NMR studies

In this study, we expressed and purified full-length NS2B from ZIKV in E. coli. Purified NS2B was then reconstituted into lyso-myristoyl phosphatidylglycerol (LMPG) micelles. It was found that compared to wild type NS2B, NS2B C11S mutation in LMPG exhibited dispersed cross peaks in the 1H-15N-HSQC spectrum, thereby suggesting the feasibility for structural characterization using solution NMR spectroscopy.
Source: Protein Expression and Purification - Category: Biochemistry Source Type: research