Towards microbial biofuel cells: Improvement of charge transfer by self-modification of microoganisms with conducting polymer – Polypyrrole

Publication date: 15 January 2019Source: Chemical Engineering Journal, Volume 356Author(s): Aura Kisieliute, Anton Popov, Roxana-Mihaela Apetrei, Geta Cârâc, Inga Morkvenaite-Vilkonciene, Almira Ramanaviciene, Arunas RamanaviciusAbstractIn this research, we are reporting the application of microorganisms modified by a conducting polymer – polypyrrole (Ppy) – for the improvement of microbial biofuel cells. The synthesis of polypyrrole was assisted by metabolic and other biochemical processes that occur in microorganisms. Therefore, in this research the reported Ppy formation method can be recognized as environmentally friendly. During herein reported synthesis of Ppy, the cell walls and some other structures of Aspergillus niger and Rhizoctania sp selected for this research were modified by the formed Ppy, which facilitated the transfer of electrical charge generated by microorganisms towards an electrode. Two electrochemical methods: (i) amperometric measurements at constant potential and (ii) scanning electrochemical microscopy (SECM) were applied for the evaluation of charge transfer efficiency and for the determination of electrochemical activity of Ppy-modified and non-modified cells. Two mediators (1,10-phenanthroline-5,6-dione (PD) or 9,10-phenanthrenequinone (PQ) and potassium ferricyanide (K3[Fe(CN)6])) based redox system was applied for the assessment of charge transfer from above mentioned living cells. The amperometric signals registered for electrodes based ...
Source: Chemical Engineering Journal - Category: Chemistry Source Type: research