An enzyme-modified capillary as a platform for simultaneous fluorometric detection of D-glucose and L- lactate

Publication date: Available online 19 September 2018Source: Journal of Pharmaceutical and Biomedical AnalysisAuthor(s): Atsushi Shoji, Yusuke Takahashi, Saki Osato, Masao SugawaraAbstractThe preparation of a glass capillary pattered with lipid layers on which lactate dehydrogenase (LDH) and glucose dehydrogenase (GDH) were regionally adsorbed and its application for simultaneous detection of D-glucose and L-lactate in human serum is described. A lipid layer was formed on the surface of BSA-unabsorbed octadecyltrichlorosilane (OTS) inner wall of a glass capillary. The electrostatic charge of the lipid layer was a key factor for adsorbing the enzymes on the lipid layer. The fluorescence intensities were observed at each enzyme site in the presence of diaphorase (DIA), β-nicotinamide-adenine dinucleotide oxidized (NAD), resazurin, D-glucose and L-lactate. The fluorescence intensities at each enzyme site increased with an increase in the concentration of D-glucose and L-lactate=with the detection limits of 32 μM and 4.9 μM, respectively.
Source: Journal of Pharmaceutical and Biomedical Analysis - Category: Drugs & Pharmacology Source Type: research

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Source: Journal of Advanced Research - Category: Research Source Type: research
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Source: Medscape Pulmonary Medicine Headlines - Category: Respiratory Medicine Tags: Infectious Diseases News Source Type: news
Suresh K. Mukherji
Source: Neuroimaging Clinics - Category: Radiology Source Type: research
SURESH K. MUKHERJI, MD, MBA, FACR
Source: Neuroimaging Clinics - Category: Radiology Source Type: research
Magnetoencephalography
Source: Neuroimaging Clinics - Category: Radiology Source Type: research
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Source: Neuroimaging Clinics - Category: Radiology Source Type: research
NEUROIMAGING CLINICS OF NORTH AMERICA
Source: Neuroimaging Clinics - Category: Radiology Authors: Source Type: research
Source: Neuroimaging Clinics - Category: Radiology Source Type: research
Resting state functional connectivity (RSFC) has been widely studied in functional magnetic resonance imaging (fMRI) and is observed by a significant temporal correlation of spontaneous low-frequency signal fluctuations (SLFs) both within and across hemispheres during rest. Different hypotheses of RSFC include the biophysical origin hypothesis and cognitive origin hypothesis, which show that the role of SLFs and RSFC is still not completely understood. Furthermore, RSFC and age studies have shown an “age-related compensation” phenomenon. RSFC data analysis methods include time domain analysis, seed-based correl...
Source: Neuroimaging Clinics - Category: Radiology Authors: Source Type: research
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Source: European Journal of Radiology - Category: Radiology Source Type: research
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