Association between the polymorphisms of angiotensin converting enzyme (Peptidyl-Dipeptidase A) INDEL mutation (I/D) and Angiotensin II type I receptor (A1166C) and breast cancer among post menopausal Egyptian females

Publication date: September 2014 Source:Alexandria Journal of Medicine, Volume 50, Issue 3 Author(s): Rania Mohamed El Sharkawy , Ahmed Mohamed Zaki , Amal Abd El Fattah Kamel , Rania Nabil Bedair , Ahmed Saad Ahmed Renin angiotensin system (RAS) is involved in the regulation of cardiovascular homeostasis. Angiotensin (Ang II) is converted from angiotensin I via angiotensin converting enzyme (ACE). Ang II exerts its effects by binding to two types of receptors; AT1R and AT2R. Ang II effect on AT1R promotes proliferation, angiogenesis and metastasis in breast tissues. ACE (I/D) polymorphism is an insertion/deletion of a 287bp DNA fragment within intron 16 of ACE gene. A1166C is a single nucleotide polymorphism (SNP) in the 3′-UTR of AT1R gene. Both (D) and (C) alleles were found to be related to RAS overactivation. Subjects and methods One hundred and twenty postmenopausal Egyptian females were included in the present study and were divided into control group (fifty apparently healthy women) and patients group (seventy breast cancer patients). Detailed history taking was done with stress on age, family history, menstrual, obstetric, medical and drug history. Physical examination including body mass index calculation was done. Histopathological examination was done for tumor grading and staging. Detection of ACE gene (I/D) polymorphism by PCR and AT1R A1166C SNP using PCR/RFLP were done. Results A statistically significant difference in AT1R A1166C SNP genotype f...
Source: Alexandria Journal of Medicine - Category: Journals (General) Source Type: research