One-step real-time PCR assay for detection and quantification of RNA HCV to monitor patients under treatment in Brazil

Publication date: Available online 20 September 2018Source: The Brazilian Journal of Infectious DiseasesAuthor(s): Elisabete Andrade, Daniele Rocha, Marcela Fontana-Maurell, Elaine Costa, Marisa Ribeiro, Daniela Tupy de Godoy, Antonio G.P. Ferreira, Amilcar Tanuri, Patrícia Alvarez, Rodrigo BrindeiroAbstractThe Brazilian Public Health Service provides pegylated alfa-interferon (αPEG-IFN) free-of-charge to treat patients infected with HCV. The primary goal of HCV therapy is the long-term elimination of HCV from the blood to reduce the risk of HCV associated complications and death. Patient viremia affects treatment duration and response, thus influencing clinical decisions. We developed a high-throughput method to perform the quantification of RNA hepatitis C virus (HCV) virus load in plasma samples to monitor patients under treatment. The method is based on a duplex detection, in a one-step real-time RT-PCR assay which has been validated according to the rules established by the official Brazilian regulatory agency (ANVISA). This new method was compared to a commercial kit (Cobas/Taqman HCV Test v2.0 – Roche), showing virus load results with significant correlation between them (p = 0.012) using commercial and clinical panels. In addition, 611 samples from patients treated with αPEG-IFN from different regions of Brazil were analyzed. Our one-step real-time RT-PCR assay demonstrated good performance in viral load measurement and in treatment course monitoring, w...
Source: The Brazilian Journal of Infectious Diseases - Category: Infectious Diseases Source Type: research