The effect of sildenafil on rats with adenine—Induced chronic kidney disease

Publication date: December 2018Source: Biomedicine & Pharmacotherapy, Volume 108Author(s): Badreldin H. Ali, Mohammed Al Za'abi, Sirin A. Adham, Yousuf Al Suleimani, Turan Karaca, Priyadarsini Manoj, Jamila Al Kalbani, Javid Yasin, Abderrahim NemmarAbstractThe erectile dysfunction drug sildenafil has cardiopulmonary protective actions, and a nephroprotective action in cisplatin and ischemia-reperfusion–induced acute kidney injury. Here, we assessed its possible ameliorative action in a model of chronic kidney disease (CKD) using adenine feeding. Eight groups of rats were treated with saline (controls), adenine (0.25% w/w in feed daily for 5 weeks), and oral sildenafil (0.1, 0.5 or 2.5 mg/kg), either alone, or concomitantly with adenine. Urine was collected 24 h after the end of the treatments from all rats and blood pressure measured, followed by collection of blood and kidneys for the measurement of several functional, biochemical and histopathological parameters. Adenine treatment reduced body weight, creatinine renal clearance, and increased water intake and urine output, as well as the plasma concentrations of urea and creatinine, neutrophil gelatinase-associated lipocalin, and N-acetyl-β-D-glucosaminidase activity, and albumin in urine. Adenine also increased the concentrations of the uremic toxins indoxyl sulfate, uric acid and phosphate, and a number of proteins and inflammatory cytokines, and decreased that of several anti – oxidant indices. Renal histopathol...
Source: Biomedicine and Pharmacotherapy - Category: Drugs & Pharmacology Source Type: research