Simultaneous electrochemical evaluation of ascorbic acid, epinephrine and uric acid at disposable pencil graphite electrode: Highly sensitive determination in pharmaceuticals and biolocigial liquids by differential pulse voltammetry.

In this study, pencil graphite electrode (PGE) was used for the simultaneous determination of ascorbic acid (AA), epinephrine (EP) and uric acid (UA) by differential pulse voltammetry (DPV) and cyclic voltammetry (CV) methods. The electrochemical oxidation peaks of, AA, NE and UA in their mixture can be well separated at about +0.15 V, +0.36 V and +0.48 V, respectively, in 0.1 M Britton-Robinson (BR) buffer solution at pH 4.0. The effects of various experimental parameters such as pH, scan rate and voltammetric parameters on the voltammetric response of these compounds were investigated. Under optimum conditions, linear calibration graphs were obtained over the AA, EP and UA concentration range to 300-900 nM, 50-150 nM and 75-195 nM, respectively. The detection limits for AA, EP and UA were found to be 50 nM, 10 nM and 15 nM in the form of mixture at the PGE. This electrode shows good analytical performance characteristics corresponding repeatability and recovery for the simultaneous determination of these compounds. For the first time in this developed method, PGE was successfully applied to test AA and UA in biological fluids and to detect AA and EP in pharmaceutical drugs. PMID: 30215332 [PubMed - as supplied by publisher]
Source: Combinatorial Chemistry and High Throughput Screening - Category: Chemistry Authors: Tags: Comb Chem High Throughput Screen Source Type: research