Cancers, Vol. 10, Pages 326: Chromogenic Multiplex Immunohistochemistry Reveals Modulation of the Immune Microenvironment Associated with Survival in Elderly Patients with Lung Adenocarcinoma

Cancers, Vol. 10, Pages 326: Chromogenic Multiplex Immunohistochemistry Reveals Modulation of the Immune Microenvironment Associated with Survival in Elderly Patients with Lung Adenocarcinoma Cancers doi: 10.3390/cancers10090326 Authors: Marius Ilié Mélanie Beaulande Saima Ben Hadj Emmanuel Chamorey Renaud Schiappa Elodie Long-Mira Sandra Lassalle Catherine Butori Charlotte Cohen Sylvie Leroy Olivier Guérin Jérôme Mouroux Charles-Hugo Marquette Jean-François Pomerol Gilles Erb Véronique Hofman Paul Hofman With underrepresentation of elderly patients with lung adenocarcinoma (LADC) in anti-PD-1/PD-L1 clinical trials, better understanding of the interplay of PD-L1 and tumor-associated immune cells (TAICs) could assist clinicians in stratifying these patients for immunotherapy. One hundred and one patients with LADCs, stratified by age, were included for analysis of PD-L1 expression and density of TAICs expressing CD4, CD8, and CD33, by using multiplex chromogenic immunohistochemistry (IHC) assays and automated digital quantification. The CD4+/CD8+ ratio was significantly higher in elderly patients. In patients <75 years, the density of CD4+, CD8+, and PD-L1 in TAICs showed a positive significant correlation with PD-L1 expression in tumor cells (TCs), while a lower correlation was observed in the elderly population. In the latter, a high CD4+/CD8+ ratio, and combined PD-L1 expression ≥1% TCs with a low CD8+ den...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research