Polymerization of Dopamine Catalyzed by Laccase: Comparison of Enzymatic and Conventional Methods

Publication date: Available online 10 September 2018Source: Enzyme and Microbial TechnologyAuthor(s): Fan Li, Yuanyuan Yu, Qiang Wang, Jiugang Yuan, Ping Wang, Xuerong FanAbstractPolydopamine (PDA) films are generally produced by spontaneous oxidation of dopamine in basic medium, which is a highly convenient method for surface modification. This method is, however, not suitable for alkaline sensitive materials, which have poor stability in alkali medium and need over-long reaction time. Herein, we compared two dopamine polymerization methods: conventional method (induced by O2 at pH 8.5) and laccase-catalyzed method (at pH 5.5). The comparison showed that the polymerization of dopamine was greatly improved when catalyzed by laccase. The PDA film obtained from laccase-catalyzed method was more uniform and more stable compared with that obtained from the conventional method; the film was able to further react with amine-terminated molecules. As a result of laccase, ether bonds were found between dopamine units of PDA; while such bonds were not observed in film obtained from the conventional method. This work can be a basis for a better understanding of structure and property relationships of PDA coating.
Source: Enzyme and Microbial Technology - Category: Biotechnology Source Type: research
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