GSE116224 Small RNA-seq analysis shows reduced tRNA levels in cdk1-as1 mutants

Contributors : Jorrit Enserink ; Carmen M HerreraSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Saccharomyces cerevisiaePurpose: Determine whether Cdk1 only regulates a specific subset of tDNAs, or whether Cdk1 has a more global impact on tDNA expression; Method: We study the expression of tRNA genes in WT cells and cdk1-as1 mutants during S phase by small-RNAseq; Result: We found that expression of at least 77% of all tDNAs is significantly downregulated in S-phase in the cdk1-as1 mutant compared with the WT strain; Conclussions: Cdk1 promotes transcription of the majority of tRNA genes during the cell cycle.

Source: GEO: Gene Expression Omnibus - September 10, 2018 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Saccharomyces cerevisiae Source Type: research