Advances in Instrumentation and Methodology for Solid-State NMR of Biological Assemblies

Publication date: Available online 8 September 2018Source: Journal of Structural BiologyAuthor(s): Rachel W. Martin, John E. Kelly, Jessica I. KelzAbstractMany advances in instrumentation and methodology have furthered the use of solid-state NMR as a technique for determining the structure and studying the dynamics of molecules involved in complex biological assemblies. Solid-state NMR does not require large crystals, has no inherent size limit, and with appropriate isotopic labeling schemes, supports solving one component of a complex assembly at a time. It is complementary to cryo-EM, in that it provides local, atomic-level detail that can be modeled into larger-scale structures. This review focuses on the development of high-field MAS instrumentation and methodology; including probe design, benchmarking strategies, labeling schemes, and experiments that enable the use of quadrupolar nuclei in biomolecular NMR. Current challenges facing solid-state NMR of biological assemblies and new directions in this dynamic research area are also discussed.

Source: Journal of Structural Biology - September 9, 2018 Category: Biology Source Type: research