Effect of Paclitaxel-Loaded PLGA Nanoparticles on MDA-MB Type Cell Lines; Apoptosis and Cytotoxicity Studies.

Effect of Paclitaxel-Loaded PLGA Nanoparticles on MDA-MB Type Cell Lines; Apoptosis and Cytotoxicity Studies. Curr Pharm Des. 2018 Sep 02;: Authors: Vijayan V, Shalini K, Yugesvaran V, Yee TH, Balakrishnan S, Palanimuthu VR Abstract Triple-Negative Breast Cancer is an aggressive type of breast cancer, unable to treat by using chemotherapy drugs, due lack of Estrogen Receptor (ER), Progesterone Receptor (PR) expression and Human Epidermal Growth Factor Receptor 2 (HER2) on the cell surface. The aim of this study was to compare the effect of paclitaxel-loaded PLGA nanoparticle (PTX-NPs) on the cytotoxicity and apoptosis of the different MDA-MB type of cell lines. PTX-NPs were prepared by nanoprecipitation method and characterized earlier. The cytotoxicity of PTX-NPs was evaluated by MTT and LDH assay, later apoptosis was calculated by flow cytometry analysis. The prepared NP size of 317.5nm and zeta potential of -12.7mV was showed drug release of 89.1% at 48 h. MDA-MB-231 type cell showed significant cytotoxicity by MTT method of 47.4±1.2% at 24 h, 34.6±0.8% at 48 h and 23.5±0.5% at 72 h and LDH method of 35.9±1.5% at 24 h, 25.4±0.6% at 48 h and 19.8±2.2% at 72 h with apoptosis of 47.28±0.42%. We have found that PTX-NPs showed the cytotoxic effect on all the MDA-MB cancer cell lines, but showed potent anticancer activities against MDA-MB-231 cell line via induction of apoptosis. PMID: 30179118 [PubMed - as supplied by pu...
Source: Current Pharmaceutical Design - Category: Drugs & Pharmacology Authors: Tags: Curr Pharm Des Source Type: research