Septic-Induced microRNA Expression Modulations Are Linked to Angiogenesis, Vasomotion, and Hypoxia-Induced Processes.

Septic-Induced microRNA Expression Modulations Are Linked to Angiogenesis, Vasomotion, and Hypoxia-Induced Processes. Adv Exp Med Biol. 2018;1072:227-231 Authors: Schmidt B, Roessler C, Schumann J Abstract Sepsis is an exaggerated immune reaction to an infection, which leads to organ dysfunction especially circulatory failure. This is based on cellular processes, which are regulated by post-transcriptional gene expression modulations including microRNAs (miRNAs). In order to elucidate the role miRNAs play in septic processes, monocytes and endothelial cells were grown in an inflammatory milieu. In addition, aortas from septic mice were investigated. Expression of miRNAs was analysed by both next generation sequencing (NGS) and NanoString technology, and miRNA targets were identified by in silico analysis. Clear alterations in miRNA expression profiles were found in monocytes, endothelial cells, and aortas exposed to septic conditions compared to the respective control. In silico analysis revealed several of the differentially expressed miRNAs to be involved in cellular response to hypoxia. In endothelial cells, for instance, miR-21-5p and miR-106b-5p emerged, which are known to interact with hypoxia inducible factor 1 alpha (HIF-1α), a major player in the process of angiogenesis. In line with this, in aortas expression changes were observed for miR-144-3p, which targets HIF-1α as well. Further validated target genes of differential...
Source: Adv Data - Category: Epidemiology Authors: Tags: Adv Exp Med Biol Source Type: research