Polyunsaturated Fatty Acids Induce ROS Synthesis in Microvascular Endothelial Cells.

Polyunsaturated Fatty Acids Induce ROS Synthesis in Microvascular Endothelial Cells.
Adv Exp Med Biol. 2018;1072:393-397
Authors: Trommer S, Leimert A, Bucher M, Schumann J
Abstract
In sepsis, endothelial dysfunction is a crucial driver known to limit the survival rate of affected patients. For this, ROS-mediated signaling plays an important role in endothelial communication and functionality. In the management of sepsis, polyunsaturated fatty acids (PUFA) have received increasing attention regarding their anti-inflammatory potential neglecting the oxidative properties of these substances. Therefore, in the present study we examined the capacity of PUFA to interfere with the expression of major ROS-producing enzymes, as well as endothelial ROS production itself. The human microvascular endothelial cells TIME (ATCC number: CRL-4025) were used. Cells were cultured in medium enriched with LNA (C18:3n3), EPA (C20:5n3), DHA (C22:6n3), LA (C18:2n6), or AA (C20:4n6) in concentrations of 15 μM totaling 144 h. Stimulation of cells was performed in the last 24 h of fatty acid supplementation by addition of the cytokines TNF-α + IL-1β + IFN-γ (5 ng/ml each). Gene expression of eNOS, COX-2, and NOX-4 was evaluated by qPCR. ROS synthesis was analyzed by means of a flow cytometry-based rhodamine 123 assay. Cytokine stimulation was found to differentially affect gene expression of major ROS synthesizing enzymes: eNOS was decreased where...

Source: Adv Data - September 5, 2018 Category: Epidemiology Authors: Trommer S, Leimert A, Bucher M, Schumann J Tags: Adv Exp Med Biol Source Type: research