GSE112920 IL-27 in vaccine elicited cellular immunity

Contributors : Ross M Kedl ; Trevor Blain ; Thomas Danhorn ; Walton KendraSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculus5000 congenically marked (CD45.1) WT or IL-27R-/- OT1 T cells were transferred into WT B6 hosts (CD45.2). One day later, the mice were immunized with ovalbumin (100 µg), PolyIC (40 µg) and anti-CD40 antibody (FGK4.5, 40 µg). 3 days after immunizayion, spleens were harvested and the OT1 T cells isolated by flow sorting based on CD45.1 expression. Each sample is the result of pooling T cells from 3 individual mice. RNA was isolated from the sorted cells and RNAseq performed.

Source: GEO: Gene Expression Omnibus - September 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research