GSE114152 Transcriptomic analysis of engineered and improved Pichia pastoris for S-adenosyl-l-methionine (SAM) production

Contributors : Jinagchao Qian ; Xiulin QinSeries Type : Expression profiling by arrayOrganism : Komagataella phaffii GS115 ; Saccharomyces cerevisiaeSAM is the principal methyl group donor in all living organisms, and has attracted much interest in clinical research. We improved SAM production in engineered P. pastoris strain GS115/DS56 by overexpression of the recombinant methionine adenosyltransferase (MAT) gene DS56, and further enhanced SAM prduction in G12-CBS by downregulation of the cystathionine β-synthase (CBS) gene CYS4 with a weak promoter G12. We performed the pairwise transcriptome comparisons between high-producing (HP) and low-producing (LP) strains, in order to understand the impact of SAM accumulation on the P. pastoris physiology and to identify genome-wide targets for further im proving SAM production.

Source: GEO: Gene Expression Omnibus - September 1, 2018 Category: Genetics & Stem Cells Tags: Expression profiling by array Komagataella phaffii GS115 Saccharomyces cerevisiae Source Type: research