Oleanolic Acid, A Novel Endothelin a Receptor Antagonist, Alleviated High Glucose-Induced Cardiomyocytes Injury.
Oleanolic Acid, A Novel Endothelin a Receptor Antagonist, Alleviated High Glucose-Induced Cardiomyocytes Injury.
Am J Chin Med. 2018 Aug 27;:1-15
Authors: Wu D, Zhang Q, Yu Y, Zhang Y, Zhang M, Liu Q, Zhang E, Li S, Song G
Abstract
Endothelin-1 (ET-1) and its receptor endothelin A receptor (ET[Formula: see text] have been shown to be upregulated in a high glucose environment, which increase the incidence of diabetes-related heart failure. Our previous study demonstrated that oleanolic acid (OA), a natural compound found in Chinese herbs had ET-1 antagonistic effects. We aimed to verify whether OA could ameliorate diabetes mellitus (DM)-induced injury in cardiomyocytes by reducing the antagonistic effects of the ET-1 pathway. For the induction of high glucose-related injury in cardiomyocytes, neonatal rat ventricular cardiomyocytes (NRVMs) were subjected to culture medium containing 25[Formula: see text]mM of glucose. Natriuretic peptide B (BNP), mitochondrial membrane potential (MMP) and cell surface area were measured to evaluate the severity of NRVMs injury. mRNA expression of ET-1 and ETA was determined using quantitative PCR. Moreover, a Ca[Formula: see text] influx assay was used to evaluate potential ETA antagonistic effects. Molecular docking of OA and ETA was performed using the Sulflex-Dock program. Human induced pluripotent stem cell (iPS-C)-derived cardiomyocytes and real time cell analysis system (RTCA) were used to verif...
Source: The American Journal of Chinese Medicine - Category: Complementary Medicine Authors: Wu D, Zhang Q, Yu Y, Zhang Y, Zhang M, Liu Q, Zhang E, Li S, Song G Tags: Am J Chin Med Source Type: research
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