Structure of the Bacterial Community in Different Stages of Early Childhood Caries

Ximenes, Marcos / Armas, Rafael Dutra de / Triches, Thaisa Cez ária / Cardoso, Mariane / Vieira, Ricardo de SouzaPage 369 - 373Purpose: To characterise in vivo the structure of bacterial communities in decayed and sound primary teeth.Materials and Methods: Samples of biofilms were collected from three groups of patients with complete and exclusively primary dentition (n = 45): G1: sound teeth (n = 15); G2: enamel lesion (n = 15); G3: dentin lesion (n = 15). DNA was extracted (CTAB 2%) from the biofilm, the partial 16S rRNA gene was amplified with Bacteria Universal Primers (BA338fGC - UN518r) and subjected to DGGE (denaturing gradient gel electrophoresis). Multidimensional scaling and ANOSIM (analysis of similarity) were employed to determine the structure of the bacterial communities. The amplicon richness was determined by averaging amplicons, with the differences between treatments determined with ANOVA, while means were compared using Tukey's test (p

Source: Oral health and Preventive dentistry - August 30, 2018 Category: Dentistry Source Type: research