Cationic Hyperbranched Polymers with Biocompatible Shells for siRNA Delivery.

Cationic Hyperbranched Polymers with Biocompatible Shells for siRNA Delivery.
Biomacromolecules. 2018 Aug 27;:
Authors: Li S, Omi M, Cartieri F, Konkolewicz D, Mao G, Gao H, Averick SE, Mishina Y, Matyjaszewski K
Abstract
Cationic hyperbranched polymers (HBP) were prepared by self-condensing vinyl polymerization of an atom transfer radical polymerization (ATRP) inimer containing a quaternary ammonium group. Two types of biocompatible shells, poly(oligoethylene glycol) methacrylate (polyOEGMA) and poly(2-(methylsulfinyl) ethyl methacrylate) (polyDMSO), were grafted respectively from HBP core to form core-shell structures with low molecular weight dispersity and high biocompatibility, polyOEGMA-HBP and polyDMSO-HBP. Both of the structures showed low cytotoxicity and good siRNA complexing ability. The efficacy of gene silencing against Runt-related transcription factor 2 ( Runx2) expression and the long-term assessment of mineralized nodule formation in osteoblast cultures were evaluated. The biocompatible core-shell structures were crucial to minimizing undesired cytotoxicity and nonspecific gene suppression. polyDMSO-HBP showed higher efficacy of forming polyplexes than polyOEGMA-HBP due to shell with lower steric hindrance. Overall, the gene silencing efficiency of both core-shell structures was comparable to commercial agent Lipofectamine, indicating long-term potential for gene silencing to treat heterotopic ossification (HO).
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Source: Biomacromolecules - August 27, 2018 Category: Biochemistry Authors: Li S, Omi M, Cartieri F, Konkolewicz D, Mao G, Gao H, Averick SE, Mishina Y, Matyjaszewski K Tags: Biomacromolecules Source Type: research