Farnesoid X Receptor Activation Modulates Calcium Homeostasis in Rat Aortic Vascular Smooth Muscle Cells.

Farnesoid X Receptor Activation Modulates Calcium Homeostasis in Rat Aortic Vascular Smooth Muscle Cells.
Chin J Physiol. 2018 Aug 24;61
Authors: Zhang R, Peng L, Ran H, Fan Y, Zhao Y, Cao F
Abstract
The farnesoid X receptor (FXR) is a member of the nuclear receptor superfamily that is expressed in
the vasculature; our previous work showed that FXR regulated vascular reactivity through NO mechanism.
The underlying mechanism for the regulation of vascular tension by FXR remains unclear. The present work
was designed to investigate whether FXR regulates calcium homeostasis in aortic vascular smooth muscle
cells (VSMCs). Protein abundances of angiotensin II type 1 and 2 receptors (AT₁R, AT₂R), bradykinin
type 1 and 2 receptors (B₁R, B₂R), and the inositol 1,4,5-trisphosphate receptor (IP₃R) were analyzed by
western blotting. Kallikrein activity and bradykinin content were assayed by using spectrophotometry
and a bradykinin assay kit, respectively. Aortic contraction, intracellular Ca²⁺ concentrations ([Ca²⁺]i),
sarco/endoplasmic reticulum Ca²⁺ ATPase (SERCA) activities, and IP₃-evoked Ca²⁺ release were investigated,
following FXR activation in the presence or absence of AT₂R and B₂R blockade. We found that the FXR
agonists GW4064 and INT-747 increased the protein abundance of AT₂R and B₂R in rat aortic VSMCs.
AT₂R blockade with PD123319 reversed the effects of FXR agonists on kallikrein activity, B₂R,...

Source: The Chinese Journal of Physiology - August 26, 2018 Category: Physiology Tags: Chin J Physiol Source Type: research