Establishment of TUSMi004-A, an induced pluripotent stem cell (iPSC) line from a 32-year old Chinese Han patient with Obsessive-Compulsive Disorder (OCD)

Publication date: Available online 24 August 2018Source: Stem Cell ResearchAuthor(s): Ying Wang, Chuanyuan Kang, Hongxiang Yu, Jing Fen, Xiquan Ma, Ying Lei, Jian ZhaoAbstractA 32-year old Obsessive-Compulsive Disorder (OCD) male patient donated his Peripheral blood mononuclear cells (PBMC). The non-integrating episomal vector system used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of transgene-free iPSCs was confirmed by immunocytochemistry for pluripotency markers and by the ability of the iPSCs to differentiate spontaneously into 3 germ layers in vitro. In addition, the iPSC line displayed a normal karyotype. Our model might offer a good platform to further study the pathological mechanisms, to identify early biomarkers, and also for drug testing studies in OCD.Resource Table.Unique stem cell line identifierTUSMi004-AAlternative name(s) of stem cell lineXWInstitutionTongji University School of Medicine, Shanghai, Peoples R ChinaContact information of distributorYing Wang, Type of cell lineiPSCOriginHumanAdditional origin infoAge: 32Sex: maleEthnicity: Chinese HanCell SourcePeripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingTransgene free, Episomal plasmidsGenetic ModificationNOType of ModificationN/AAssociated diseaseObsessive-Compulsive Disorder(OCD)Gene/locus5-HT1A/chr 5:63258565, rs6295 C-G,LHPP/chr 10:126244970, rs35936514 C-T,HTR1A/chr 63,255,991, rs878567 C-TMethod of modificationN/AName of transgene or ...
Source: Stem Cell Research - Category: Stem Cells Source Type: research