Down-regulation of microRNA-429 contributes to angiotensin II-induced profibrotic effect in rat kidney.

Down-regulation of microRNA-429 contributes to angiotensin II-induced profibrotic effect in rat kidney. Am J Physiol Renal Physiol. 2018 Aug 22;: Authors: Wang Z, Zhu Q, Wang W, Hu J, Li PL, Yi F, Li N Abstract MicroRNA (miR) 429 has been shown to inhibit epithelial-to-mesenchymal transition (EMT) in cancer cells. However, the role of miR429 in EMT in non-cancer cells has not been defined, especially in the kidneys. The present study determined whether miR429 participated in angiotensin (ANG) II-induced EMT and fibrogenesis in renal cells. In NRK-52E cells, a rat proximal tubular cell line, incubation of ANG II (10-9 M) for 24 h significantly reduced the level of miR429 by 60%, and meanwhile increased the protein levels of mesenchymal markers α-smooth muscle actin and fibroblast-specific protein-1 by 3 fold and decreased epithelial marker E-cadherin by 60%, which was blocked by Losartan, a AT1 receptor antagonist. Treatment of cells with miR429 inhibitor produced the similar changes in the above EMT markers to that induced by ANG II. In cells overexpressed with miR429 transgene, ANG II-induced increases in collagen were abolished. Male Sprague-Dawley rats were infused with ANG II (200 ng/kg/min) for 12 days and the levels of miR429 in the kidneys were reduced by 75%. Intrarenal transfection of lentivirus expressing miR429 also reversed the ANG II-induced changes in the EMT markers and collagen in the kidneys. The ANG II-induced incr...
Source: Am J Physiol Renal P... - Category: Urology & Nephrology Authors: Tags: Am J Physiol Renal Physiol Source Type: research