The exoproteome profiles of three Staphylococcus saprophyticus strains reveal diversity in protein secretion contents

Publication date: Available online 23 August 2018Source: Microbiological ResearchAuthor(s): Andrea Santana de Oliveira, Isabella Inês Rodrigues Rosa, Evandro Novaes, Lucas Silva de Oliveira, Lilian Cristiane Baeza, Clayton Luiz Borges, Lennart Marlinghaus, Célia Maria de Almeida Soares, Marcia Giambiagi-deMarval, Juliana Alves Parente-RochaAbstractStaphylococcus saprophyticus is a gram-positive microorganism responsible for urinary tract infections (UTIs). Although some virulence factors are characterized, such as urease, autolysins, adhesins and hemagglutinins, large-scale proteomic studies have not been performed within this species. We performed the characterization of the exoproteome from three S. saprophyticus strains: the reference strain ATCC 15305, a non-capsular strain 7108 and the 9325 strain containing a thick capsule which were cultured in BHI medium and culture supernatants were analysed by using mass spectrometry approach. We observed a core of 72 secreted proteins. In addition, it was possible to detect diversity in the protein profiles of the exoproteomes. Interestingly, strain 7108 presented no secretion of three antigenic proteins, including the classical SsaA antigen. In addition, the level of antigenic proteins secreted by strain 9325 was higher than in ATCC 15305. This result was confirmed by Western blot analysis using anti-SsaA polyclonal antibodies, and no production/ secretion of SsaA was detected in strain 7108. Transcriptional data shows that 7108...
Source: Microbiological Research - Category: Infectious Diseases Source Type: research