Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast.

Comparative proteomics of soluble factors secreted by human breast adipose tissue from tumor and normal breast. Oncotarget. 2018 Jul 24;9(57):31007-31017 Authors: Fletcher SJ, Hapon MB, Callegari EA, Crosbie ML, Santiso N, Ursino A, Amato AR, Gutiérrez A, Sacca PA, Dreszman R, Pérez A, Carón RW, Calvo JC, Pistone-Creydt V Abstract Tumor progression depends on the tumor-stroma interaction. In the breast, adipose tissue is the predominant stromal type. We have previously demonstrated that conditioned media (CMs) from explants of human adipose tissue of tumor breasts (hATT) increase proliferation and migration of breast cancer epithelial cells when compared to human adipose tissue from normal breasts (hATN). In this work, we aim to identify specific proteins and molecular/biological pathways associated with the secretion profile of hATT and hATN explants. hATT-CMs and hATN-CMs were separated by SDS-PAGE and analyzed by means of two-dimensional nano-liquid chromatography-mass spectrometry. The data was analyzed using ProteoIQ and FunRich software. In addition, 42 cytokines from hATT-CMs and hATN-CMs were assayed by a protein antibody assay. Compared to hATN-CMs, hATT-CMs showed greater protein diversity. We found that hATT-CMs presented a greater amount of proteins related to complement system activity, metabolism and immune system, as well as proteins involved in a variety of biological processes such as signal transduction and cell ...
Source: Oncotarget - Category: Cancer & Oncology Tags: Oncotarget Source Type: research