Oncogene miR-154-5p regulates cellular function and acts as a molecular marker with poor prognosis in renal cell carcinoma

Publication date: Available online 21 August 2018Source: Life SciencesAuthor(s): Canbin Lin, Zuwei Li, Peijie Chen, Jing Quan, Xiang Pan, Liwen Zhao, Liang Zhou, Yulin Lai, Tao He, Weijie Xu, Jinling Xu, Xin Guan, Hang Li, Shangqi Yang, Yimin Hu, Yongqing LaiAbstractAimsIn adult population, the renal cell carcinoma (RCC) is one of the most common urological malignancies. It is meaningful to research for the molecular markers which are involved in the occurrence and development of RCC. Therefore, we concentrate on illuminating the role of microRNA-154-5p in progression of RCC and explore its prognostic values.Main methodsThe real-time quantitative polymerase chain reaction (RT-qPCR) was applied to determine expression level of miR-154-5p in tissues. Afterwards, the transfected cell lines ACHN and 786-O were used for the CCK-8 assay, MTT assay, wound healing assay, transwell assay and flow cytometric assay to explore the role of miR-154-5p in regulating cellular function. In addition, formalin-fixed paraffin-embedded (FFPE) renal cancer samples were used for detecting the relationship between expression level of miR-154-5p and clinical information. Furthermore, univariate and multivariate Cox proportional-hazards regression analyses, and the Kaplan-Meier survival curves were performed to evaluate the prognostic value of miR-154-5p in RCC.Key findingsThe RT-qPCR indicated that miR-154-5p is up-regulated in RCC pathologic specimens and cell lines. Results of study also demonstrat...
Source: Life Sciences - Category: Biology Source Type: research