Tracking Tonic Dopamine Levels in vivo using Multiple Cyclic Square Wave Voltammetry

Publication date: Available online 20 August 2018Source: Biosensors and BioelectronicsAuthor(s): Yoonbae Oh, Michael L. Heien, Cheonho Park, Yu Min Kang, Jaekyung Kim, Suelen Lucio Boschen, Hojin Shin, Hyun U Cho, Charles D. Blaha, Kevin E. Bennet, Han Kyu Lee, Sung Jun Jung, Kendall H. Lee, Dong Pyo JangAbstractFor over two decades. fast-scan cyclic voltammetry (FSCV) has served as a reliable analytical method for monitoring dopamine release in near real-time in vivo. However, contemporary FSCV techniques have been limited to measuring only rapid (on the order of seconds, i.e. phasic) changes in dopamine release evoked by either electrical stimulation or elicited by presentation of behaviorally salient stimuli, and not slower changes in the tonic extracellular levels of dopamine (i.e. basal concentrations). This is because FSCV is inherently a differential method that requires subtraction of prestimulation tonic levels of dopamine to measure phasic changes relative to a zeroed baseline. Here, we describe the development and application of a novel voltammetric technique, multiple cyclic square wave voltammetry (M-CSWV), for analytical quantification of tonic dopamine concentrations in vivo with relatively high temporal resolution (10 seconds). M-CSWV enriches the electrochemical information by generating two dimensional voltammograms which enable high sensitivity (limit of detection, 0.17 nM) and selectivity against ascorbic acid, and 3,4-dihydroxyphenylacetic acid ...
Source: Biosensors and Bioelectronics - Category: Biotechnology Source Type: research