Comparison of five methods of extraction of Staphylococcus aureus DNA for molecular detection by PCR

CONCLUSIONS: Although one protocol seemed more efficient than the others, PCR was sensitive enough to allow for detection of S. aureus with all the protocols.
Source: Revista da Sociedade Brasileira de Medicina Tropical - Category: Tropical Medicine Source Type: research

Related Links:

Publication date: March 2020Source: Materials Science and Engineering: C, Volume 108Author(s): Nehal Salahuddin, Mohamed Abdelwahab, Mohamed Gaber, Sahar ElneanaeyAbstractBiodegradable, biocompatible and non-toxic polymer-based nanoparticles are the novel nanotherapeutic tool which is used for adsorption and encapsulation drugs. Extended release formulation of Norfloxacin antibiotic, chemotherapeutic agent model, drug in the form of encapsulated and loaded poly (lactic acid) nanocomposites-based Titanium dioxide (PLA/TiO2) was developed. Nanocomposites were prepared using different contents (1, 3, 5 wt %) and morphologie...
Source: Materials Science and Engineering: C - Category: Materials Science Source Type: research
Publication date: November 2019Source: Saudi Journal of Biological Sciences, Volume 26, Issue 7Author(s): Asmaa FadenAbstractWe assessed the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) strains on surfaces of hospital dental clinics. Specimens were obtained from 5 clinically symptoms-free patients of five different specialties clinics (Implantology, Pediatric Dentistry, Prosthetics, Restorative Dentistry, and Oral Medicine) of the Dental Clinic Hospital of King Saud University before and after each patient. A Q-tip swabs were used from 10 surfaces in each clinic (Arm rest of dental chair, floor beneath ...
Source: Saudi Journal of Biological Sciences - Category: Biology Source Type: research
Publication date: Available online 16 November 2019Source: Carbohydrate PolymersAuthor(s): Hossein Rahmani, Seyed Heydar Mahmoudi Najafi, Alireza Ashori, Marzieh Arab Fashapoyeh, Farzaneh Aziz Mohseni, Sara TorkamanAbstractIn this research, biocomposite films containing chitosan (CS), polyvinyl alcohol (PVA) and polyvinyl pyrrolidone (PVP), with different ratios, have been provided. The effects of adding hexamethylene 1, 6-di(aminocarboxysulfonate) (HMDACS) as cross linker and the formation of urea linkage on mechanical properties such as tensile strength and elongation and dynamic-mechanical properties such as storage mod...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 14 November 2019Source: Carbohydrate PolymersAuthor(s): Ruzanna Ahmad Shapi’i, Siti Hajar Othman, Norhazirah Nordin, Roseliza Kadir Basha, Mohd Nazli NaimAbstractChitosan nanoparticles (CNP) were synthesized via ionic gelation and used for the preparation of starch-based nanocomposite films containing different concentration of CNP (0, 5, 10, 15, 20% w/w). Antimicrobial properties of starch/CNP films was evaluated via in vitro (disc diffusion analysis) and in vivo (microbial count in wrapped cherry tomatoes) study. It was found that inhibitory zone of the 15 and 20% of starch/CNP fi...
Source: Carbohydrate Polymers - Category: Biomedical Science Source Type: research
Publication date: Available online 5 November 2019Source: Inorganica Chimica ActaAuthor(s): Mousumi Nayak, Ashish Kumar Singh, Pradyot Prakash, Rajni Kant, Subrato BhattacharyaAbstractThiosalicylato complexes of Zn(II) and Hg(II), [Zn(SC6H4CO2)(TMEDA)]2 (1) and [TMEDA-H2][Hg(SC6H4CO2)2] (2) where (TMEDA = N,N,N',N'-tetramethylethylenediamine) have been synthesized and characterized by spectral and single crystal X-ray analyses. Thiosalicylate opted different binding modes; in 1 all three binding sites are used whereas in 2 bonding of the ligands are mainly through S atoms, however, Hg-O distances indicate the existence...
Source: Inorganica Chimica Acta - Category: Chemistry Source Type: research
Abstract Milk pasteurization eliminates vegetative pathogenic microorganisms and reduces microorganisms associated with spoilage. Camel milk is a well-accepted, traditionally consumed food in Arab countries. The present study aimed to investigate the microflora of pasteurized camel milk sold in Riyadh City, Saudi Arabia. The heat resistance of the microflora was tested in culture medium and lab-sterilized milk, and its composition was verified by multiplex polymerase chain reaction (PCR) using specific primers. Further verification was performed by using separate specific primers. The identified strain survived he...
Source: Bioscience Reports - Category: Biomedical Science Authors: Tags: Biosci Rep Source Type: research
Publication date: Available online 14 November 2019Source: American Journal of Infection ControlAuthor(s): Hussein Abou Ghaddara, Jessica A. Kumar, Jennifer L. Cadnum, Yilen K. Ng-Wong, Curtis J. DonskeyIn a randomized, nonblinded, placebo-controlled trial, a single application of a 10% povidone iodine preparation significantly reduced nasal methicillin-resistant Staphylococcus aureus at 1 and 6 hours after application, but suppression was not sustained at 12 or 24 hours. Twice-daily treatment for 5 days did not reduce nasal methicillin-resistant S aureus measured 12 hours postdosing in comparison to controls. These result...
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
ConclusionsAn antibiotic allergy label was associated with significantly higher rates of MRSA colonization but no statistical difference with VRE colonization.
Source: American Journal of Infection Control - Category: Infectious Diseases Source Type: research
Molecules, Vol. 24, Pages 4151: Synthesis and Antimicrobial Activity of Methoxy- Substituted γ-Oxa-ε-lactones Derived from Flavanones Molecules doi: 10.3390/molecules24224151 Authors: Witold Gładkowski Monika Siepka Tomasz Janeczko Edyta Kostrzewa-Susłow Jarosław Popłoński Marcelina Mazur Barbara Żarowska Wojciech Łaba Gabriela Maciejewska Czesław Wawrzeńczyk Six γ-oxa-ε-lactones, 4-phenyl-3,4-dihydro-2H-1,5-benzodioxepin-2-one (5a) and its five derivatives with methoxy groups in different positions of A and B rings (5b–f), were synthesized fr...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
Authors: Kaul G, Saxena D, Dasgupta A, Chopra S Abstract Sarecycline hydrochloride (Seysara) is a novel, narrow-spectrum tetracycline derivative approved by the U.S. Food and Drug Administration (FDA) in October 2018 for the treatment of inflammatory non-nodular moderate to severe acne vulgaris. It was initially developed by Paratek Pharmaceuticals, Inc. (U.S.) and Allergan plc (U.S.), which later was acquired by Almirall S.A. (Barcelona, Spain). Almirall S.A. obtained U.S. FDA approval of oral sarecycline tablets under the trade name Seysara. Sarecycline exhibits antibacterial activity against important skin/soft ...
Source: Drugs of Today - Category: Drugs & Pharmacology Tags: Drugs Today (Barc) Source Type: research
More News: Staphylococcus Aureus | Study | Tropical Medicine