Development of a molecular serotyping scheme and a multiplexed luminex-based array for Providencia

Publication date: Available online 20 August 2018Source: Journal of Microbiological MethodsAuthor(s): Yuhui Du, Huiying Li, Zhiqiu Yin, Antoni Rozalski, Agnieszka Torzewska, Pan Yang, Chengqian Qian, Tingting Xu, Hengchun Cao, Pan Wu, Lingyan Jiang, Xi Guo, Di Huang, Bin LiuAbstractProvidencia is an opportunistic human pathogen that belongs to the Enterobacteriaceae family. The bacterial cell surface O-antigen is one of the most structurally variable cell constituents and serves as a basis for serotyping gram-negative bacteria. In this work, the genomes of 12 Providencia strains were sequenced, and genes driving O-antigen biosynthesis were analyzed. The O-antigen-synthesizing genes of Providencia are located in the O-antigen gene cluster (OGC) between the cpxA and yibK genes. The gene functions predicted in silico agreed with the known O-antigen structures. All clusters were found to contain both wzx and wzy and exhibit a high degree of heterogeneity. Based on the sero-specific genes, we developed a molecular serotyping system to detect 23 serotypes (from the present and previous studies) for the first time. Five Proteus strains, five Morganella strains, five uropathogenic Escherichia coli (UPEC) strains and 32 Providencia strains with other serotypes were used to assess the specificity of our multiplexed Luminex-based array. Five serogroups (O3, O8, O19, O38 and O52 strains) were used to determine the sensitivity of the suspension array. The detection sensitivity was 0.1 n...
Source: Journal of Microbiological Methods - Category: Microbiology Source Type: research