Microbiological evaluation of infant foods quality and molecular detection of Bacillus cereus toxins relating genes

Publication date: Available online 19 August 2018Source: Toxicology ReportsAuthor(s): Zeinab I. Sadek, Mohamed A. Abdel-Rahman, Mohamed S. Azab, Osama M. Darwesh, Mahmoud S. HassanAbstractA 205 samples representing eight different infant foods with various based materials were collected and analyzed for their microbiological properties. The contamination rate by aerobic spore formers was achieved 100 % in milk based infant food with fruit, vegetables, honey, rice and infant milk powder. While, it was detected in 95, 60 and 65% of the infant food with wheat milk based, ready to use (infant food with fruit) and ready to use (infant food with vegetables), respectively. Biochemical Identification and API 50 CHB used to identify the obtained isolates and revealed that B. subtilis was the most frequently occurring Bacillus spp. Followed by B. licheniformis and B. circulans. While B. cereus was detected in 10.20 % of the total isolates. Moreover, B. cereus was confirmed in 21.2% of milk based fruit, vegetables (15.7%), honey (17.2%), rice (14.1%) and wheat (12%) and vanished in the infant milk powder samples. Although, B. cereus noted in lower percentage but this strain is considered as the more harmful one in lower numbers. For that, the following part is focused on B. cereus. Forty five isolates obtained from B. Cereus contaminating samples were screened for prevalence of 3 important virulent enterotoxigenic genes using PCR technique. The CYTK gene had the highest presence which d...
Source: Toxicology Reports - Category: Toxicology Source Type: research

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Bacillus cereus isolates causing an outbreak in the neonatal intensive care unit were investigated using whole-genome sequencing. The outbreak coincided with construction work performed adjacent to the neonatal intensive care unit and ceased after strict sealing of the construction area. We found the outbreak to be polyclonal, however, the clonality did not correlate with the virulence in vivo. Genotypically similar isolates were associated with both lethal/severe infection and colonization/environmental contamination. Environmental bacterial load may be a major determinant of infection, especially in high-risk patients. C...
Source: The Pediatric Infectious Disease Journal - Category: Infectious Diseases Tags: Maternal-Neonatal Reports Source Type: research
Kimberly D. Gwinn Sciadopitys verticillata (Sv) produces a white, sticky, latex-like resin with antimicrobial properties. The aims of this research were to evaluate the effects of this resin (Sv resin) on bacterial populations and to determine the impact of its primary volatile components on bioactivity. The impact of sample treatment on chemical composition of Sv resin was analyzed using Fourier transform infrared spectroscopy (FTIR) coupled with principal component analysis. The presence and concentration of volatiles in lyophilized resin were determined using gas chromatography/mass spectrometry (GC/MS). Changes in...
Source: Molecules - Category: Chemistry Authors: Tags: Article Source Type: research
We describe a case of a 59-year-old male with esophageal adenocarcinoma undergoing chemotherapy presenting with a right upper lobe necrotizing pneumonia secondary to B. cereus with consequent massive hemoptysis.
Source: Respiratory Medicine Case Reports - Category: Respiratory Medicine Source Type: research
Both nosocomial pathogens, such as Streptococcus pneumoniae and Haemophilus influenzae and food-borne pathogens, such as Bacillus cereus and Clostridium perfringens are known to be detrimental to human and animal health. The effectiveness of currently used treatments for these pathogens becomes limited as resistant strains emerge. Therefore, new methods for eliminating bacterial pathogens must be developed continuously. This includes establishing novel targets to which drug discovery efforts could be focused. A promising method for discovering new drug targets in prokaryotes is to take advantage of the information availabl...
Source: Frontiers in Microbiology - Category: Microbiology Source Type: research
We report here a case of blue infectious pseudochromhidrosis caused by pigment-producing Bacillus cereus and the results of a literature review. PMID: 31612235 [PubMed - as supplied by publisher]
Source: Acta Dermato-Venereologica - Category: Dermatology Authors: Tags: Acta Derm Venereol Source Type: research
Abstract Kanosamine is an aminosugar antibiotic, and component of complex antibiotics such as kanamycin. The biosynthesis of kanosamine varies among different bacteria; best known is a pathway starting from UDP-glucose, but Bacillus subtilis can produce kanosamine in a three-step pathway from glucose 6-phosphate. A set of genes proposed to encode a kanosamine pathway has previously been identified within the zwittermicin A gene cluster of Bacillus cereus UW85. These genes, designated kabABC, are similar to the B. subtilis kanosamine pathway genes (ntdABC), but have never been studied experimentally. We have expres...
Source: Archives of Biochemistry and Biophysics - Category: Biochemistry Authors: Tags: Arch Biochem Biophys Source Type: research
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Source: Mycoscience - Category: Biology Source Type: research
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Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
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Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Tags: Article Source Type: research
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Source: Biocatalysis and Agricultural Biotechnology - Category: Biotechnology Source Type: research
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